MABT823

Anti-alpha-Actin Antibody, Cardiac Muscle Antibody, clone 22D3

clone 22D3, from mouse

• Sinonimo/i: Actin, alpha cardiac muscle 1, Alpha-cardiac actin
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 22D3, monoclonal
• Reattività contro le specie: rat, human, goat, rabbit
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), mouse (based on 100% sequence homology)
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_005150.1
• N° accesso UniProt: P68032
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ACTC1(70)

Descrizione

Descrizione generale

Actin, alpha cardiac muscle 1 (UniProt P68032; also known as Alpha-cardiac actin) is encoded by the ACTC1 (also known as ACTC, ASD5, CMD1R, CMH11, LVNC4) gene (Gene ID 70) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.

Specificità

Clone 22D3 specifically detected alpha-cardiac actin in rat myocardium homogenates with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

Immunogeno

KLH-conjugated linear peptide corresponding to human mature alpha-cardiac actin N-terminal end sequence with an acetylated N-terminus.

Applicazioni

Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed, 0.05% NP-40-permeabilzed rectangular rabbit cardiomyocytes by indirect immuoflourescence (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

Immunofluorescence Analysis: A representative lot immunostained cardiomyocytes in human left ventricular endocardial biopsies, rabbit left ventricular myocardium, as well as in goat atrial myocardium frozen tissue sections by fluorescent immunohistochemistry (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

Western Blotting Analysis: A representative lot specifically detected alpha-cardiac actin in rat myocardium homogenate with no cross-reactivity toward - or -smooth muscle actin in rat aorta and chicken gizzard homogenates, cytoplasmic - or -actin in human platelet lysate, nor -skeletal actin in rat skeletal muscle homogenate (Driesen, R.B., et al. (2009). J. Cell. Mol. Med. 13(5):896-908).

Research Category
Cell Structure

This mouse monoclonal Anti-alpha-Actin Antibody, Cardiac Muscle, clone 22D3, Cat. No. MABT823 is validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting for the detection of alpha-cardiac actin.

Qualità

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 2 µL of this antibody detected alpha-cardiac actin in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilzed HeLa cells.

Descrizione del bersaglio

41.78/42.02 kDa (mature/pro-form) calculated.

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable