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Merck
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Documenti fondamentali

MABT29

Sigma-Aldrich

Anti-Ninein Antibody, clone 79-160-7

clone 79-160-7, from mouse

Sinonimo/i:

ninein (GSK3B interacting protein), Glycogen synthase kinase 3 beta-interacting protein, ninein centrosomal protein, GSK3B-interacting protein

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

79-160-7, monoclonal

Reattività contro le specie

human

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso NCBI

NP_891989

N° accesso UniProt

Q8N4C6

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NIN(51199)

Descrizione generale

Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.

Immunogeno

Recombinant protein corresponding to human Ninein.

Applicazioni

Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).

Qualità

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.

Descrizione del bersaglio

~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa

Stato fisico

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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David K Moss et al.
Journal of cell science, 120(Pt 17), 3064-3074 (2007-08-19)
Cell-to-cell contact and polarisation of epithelial cells involve a major reorganisation of the microtubules and centrosomal components. The radial microtubule organisation is lost and an apico-basal array develops that is no longer anchored at the centrosome. This involves not only
Nathalie Delgehyr et al.
Journal of cell science, 118(Pt 8), 1565-1575 (2005-03-24)
The centrosome organizes microtubules by controlling nucleation and anchoring processes. In mammalian cells, subdistal appendages of the mother centriole are major microtubule-anchoring structures of the centrosome. It is not known how newly nucleated microtubules are anchored to these appendages. We
Ninein leads the way in vessel sprouting.
Bautch, Victoria L
Arteriosclerosis, Thrombosis, and Vascular Biology, 28, 2094-2095 (2008)
Susanne Graser et al.
The Journal of cell biology, 179(2), 321-330 (2007-10-24)
Primary cilia (PC) function as microtubule-based sensory antennae projecting from the surface of many eukaryotic cells. They play important roles in mechano- and chemosensory perception and their dysfunction is implicated in developmental disorders and severe diseases. The basal body that
Aamir Ali et al.
Nature communications, 14(1), 289-289 (2023-01-27)
Organization of microtubule arrays requires spatio-temporal regulation of the microtubule nucleator γ-tubulin ring complex (γTuRC) at microtubule organizing centers (MTOCs). MTOC-localized adapter proteins are thought to recruit and activate γTuRC, but the molecular underpinnings remain obscure. Here we show that
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