MABT201

Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51

clone SNAKA51, from mouse, purified by using protein G

• Sinonimo/i: Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: SNAKA51, monoclonal
• Purificato mediante: using protein G
• Reattività contro le specie: human
• tecniche: activity assay: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2α
• N° accesso NCBI: NP_001138472
• N° accesso UniProt: P08648
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ITGAV(3685)

Descrizione

Descrizione generale

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The α5/β1 integrin, also termed VLA5 or CD49e, is identical to the fibronectin receptor and mediates the adhesion of cells to fibronectin.

Immunogeno

Whole cells corresponding to human Integrin alpha5.

Applicazioni

Activity Assay: A representative lot of this antibody converts alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.).
Immunocytochemistry Analysis: A representative lot of this antibody detected Integin alpha 5 in Primary human foreskin fibroblasts (hFF) (Clark, K., et al. (2005). J Cell Sci. 118(Pt 2):291-300.; Valdembri, D., et al. (2009). PLoS Biol. 7(1):e25.).

Detect Integrin alpha5 (Preservative Free) using this Anti-Integrin alpha5 (Preservative Free) Antibody, clone SNAKA51 validated for use in Western Blotting, Activity Assay, ICC.

Research Category
Cell Structure

Qualità

Evaluated by Flow Cytometry in HeLa cell treated with fibronectin.
Flow Cytometry Analysis: 1 µg of this antibody detected Integrin alpha5 in 0.5X10^6 HeLa cells.

Descrizione del bersaglio

116 kDa calculated

Stato fisico

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.

Risultati analitici

Control
HeLa cells

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable