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Key Documents

MABT1558

Sigma-Aldrich

Anti-Utrophin Antibody, clone MANCHO3 (8A4)

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About This Item

Codice UNSPSC:
12352203

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

MANCHO3 (8A4), monoclonal

PM

calculated mol wt 394.47 kDa
observed mol wt ~400 kDa

Purificato mediante

using protein G

Reattività contro le specie

human, mouse

Reattività contro le specie (prevista in base all’omologia)

canine

Confezionamento

antibody small pack of 100 μg

tecniche

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG1κ

Sequenza dell’epitopo

C-terminal

N° accesso ID proteina

N° accesso UniProt

Condizioni di spedizione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... UTRN(7402)

Categorie correlate

Descrizione generale

Utrophin (UniProt: P46939; also known as Dystrophin-related protein 1, DRP-1) is encoded by the UTRN (also known as DMDL, DRP1) gene (Gene ID: 7402) in human. Utrophin is a dystrophin related protein that displays a sequence homology with dystrophin and shares many of the protein-binding properties. It plays a role in anchoring the cytoskeleton to the plasma membrane. It contains 2 calponin homology domains in its N-terminal region (aa 31-135 and 150-255) and has 22 spectrin homology repeats. The C-terminal sequence in utrophin is highly conserved between dystrophin and utrophin in most species. Utrophin is distributed throughout the sarcolemma in fetal and regenerating muscle, but is shown to be down-regulated in normal adult muscle and is restricted to the myotendinous and neuromuscular junctions. Four different isoforms of utrophin have been described that are produced by alternative promoter usage and they differ in their distribution pattern. Isoform 1 displays high expression in muscle tissue. Similar to dystrophin, utrophin can also reported to form an extensive lateral association with actin filaments and protect actin filaments from depolymerization in vitro. However, utrophin binds laterally along actin filaments through contribution of acidic spectrin-like repeats rather than the cluster of basic repeats used by dystrophin. It has been suggested that utrophin could functionally compensate for the lack of dystrophin and studied have shown that transgenic overexpression of utrophin in dystrophin-deficient mdx mice results in full recovery for all known parameters of the dystrophic phenotype. (Ref.: Sengupta, K., et al. (2020). Sci. Rep. 10; 21492; Porter, JD., et al.(1998). J. Cell Sci. 111(13); 1801-1811; Rybakova, IN., et al., (2002). Mol. Biol. Cell. 13(5); 1512-1521).

Specificità

Clone MANCHO3 (8A4) is a mouse monoclonal antibody that detects Utrophin. It targets an epitope within 42 amino acids from the C-terminal region.

Immunogeno

A recombinant fragment corresponding the 329 amino acids from the C-terminal region of human Utrophin.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Utrophin in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Utrophin in NIH3T3 cell lysate.

Immunofluorescence Analysis: A representative lot detected Utrophin in Immunofluorescence applications (McMorran, B.J., et al. (2016). Glycobiology. 26(10):1120-1132).

Immunohistochemistry Applications: A representative lot detected Utrophin in Immunohistochemistry applications (Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700).

Western Blotting Analysis: A representative lot detected Utrophin in Western Blotting applications (Morris, G.E., et al. (1998). Biochemistry. 37(31):11117-27; Nguyen T.M. et al. (1991). J Cell Biol. 115(6):1695-700; Lien, C.F., et al. (2012). J Biol Chem. 287(49):41374-85; Ghosh, T., et al. (2015). PLoS One;10(7):e0134809; Lindsay, A., et al. (2019). FEBS J.;286(13):2562-2576).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Utrophin, clone MANCHO3 (8A4), Cat. No. MABT1558, is a mouse monoclonal antibody that detects Utrophin and is tested for use in Immunofluorescence, , Immunohistochemistry, and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

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