MABS64

Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2

clone 6E5.2, from mouse

• Sinonimo/i: mitogen-activated protein kinase 14, cytokine suppressive anti-inflammatory drug binding protein, stress-activated protein kinase 2A, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, MAP kinase MXI2, MAX-interacting pro
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 6E5.2, monoclonal
• Reattività contro le specie: human, mouse
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_001306.1
• N° accesso UniProt: Q16539
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pThr180/pTyr182)
• Informazioni sul gene: human ... MAPK14(1432)

Descrizione

Descrizione generale

The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Specificità

This antibody recognizes p38 when phosphorylated at Thr180 and Tyr182.

Immunogeno

Epitope: Phosphorylated Thr180 and Tyr182

Linear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182.

Applicazioni

Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells.

Research Category
Signaling

Research Sub Category
MAP Kinases

Qualità

Evaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.

Descrizione del bersaglio

41 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Anisomycin treated and untreated HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable