MABS2314

Anti-RHBDF2 Antibody, clone RHF2B 11H7

• Sinonimo/i: Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein, iRhom2, inactive rhomboid protein 2
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 200
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: RHF2B 11H7, monoclonal
• PM: observed mol wt ~95 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: mouse, human
• Confezionamento: antibody small pack of 100
• tecniche: western blot: suitable
• Isotipo: IgG2a
• Sequenza dell’epitopo: N-terminal half
• N° accesso ID proteina: NP_001161152
• N° accesso UniProt: Q80WQ6
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: mouse ... Rhbdf2(217344)

Descrizione

Specificità

Clone RHF2B 11H7 is a rat monoclonal antibody that detects Inactive rhomboid protein 2 (RHBDF2). It targets an epitope within 15 amino acids from the N-terminal half.

Immunogeno

Ovalbumin-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of mouse Inactive rhomboid protein 2 (RHBDF2).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RHBDF2/iRhom2 in MCF-7 cell lysate.

Tested Applications

Western Blotting:

Lipopolysaccharide (LPS; 100 ng/mL; 24 h)-treated wild-type macrophage fractions from mouse bone marrow and RHBDF2 knockouts were probed with Cat. No. MABS2314, Anti-RHBDF2, clone RHF2B 11H7 (1:500 Dilution). Proteins were visualized using a Goat Anti-Rat IgG2a secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RHBDF2 (~ 98 kDa). (Data courtesy: Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich.

Western Blotting Analysis: A representative lot detected RHBDF2 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Inactive rhomboid protein 2 (UniProt: Q80WQ6; also known as iRhom2, Rhomboid family member 2, Rhomboid veinlet-like protein 6, Rhomboid-related protein) is encoded by the Rhbdf2 (also known as Irhom2, Rhbdl6, Rhor) in murine species. RHBDF2/iRhom2, a multi-pass membrane protein with seven transmembrane domains, is predominantly localized in the endoplasmic reticulum (ER) membrane. It is reported to be crucial for the maturation and function of ADAM17 in myeloid cells. Hence, mice lacking this protein are protected from ADAM17- and TNF -dependent septic shock and inflammatory arthritis. ADAM17, a sheddase of the EGF receptor ligands and TNF , is shown to be involved in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own. RHBDF2 is involved in negative regulation of protein secretion and regulation of EGF receptor signaling pathway. By interacting with EGF, it may retain EGF in the endoplasmic reticulum and regulate its degradation through the ER-associated degradation (ERAD). (Ref.: Weskamp, G., et al. (2020). J. Biol. Chem. 295(13); 4350-4358; Li, X., et al. (2017). J. Cell Sci. 130(5); 868-878; Blaydon, DC., et al. (2012). Am. J. Hum. Genet. 90(2); 340-436; Zetti, M., et al. (2011). Cell. 145(1); 79-91).

Stato fisico

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Ricostituzione

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable