58983
SUPELCOSIL™ LC-8 HPLC Column
3 μm particle size, L × I.D. 15 cm × 4.6 mm
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About This Item
Prodotti consigliati
agenzia
suitable for USP L7
Livello qualitativo
Caratteristiche
endcapped
Produttore/marchio commerciale
SUPELCOSIL™
Grado di funzionalizzazione
6.0% carbon loading
Parametri
≤70 °C temp. range
400 bar pressure (5801 psi)
tecniche
HPLC: suitable
Lungh. × D.I.
15 cm × 4.6 mm
Area superficiale
170 m2/g
Copertura della superficie
surface coverage 3.2 μmol/m2
Matrice
silica gel, spherical particle platform
Gruppo funzionale matrice
C8 (octyl) phase
Dimensione particelle
3 μm
Dimensione pori
120 Å
Intervallo di pH
2-7.5
applicazioni
food and beverages
Tecnica di separazione
reversed phase
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Descrizione generale
A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.
Note legali
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
Protocolli
HPLC Analysis of Free Fatty Acids on SUPELCOSIL™ LC-8
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