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SUPELCOSIL LC-8 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501

agenzia

suitable for USP L7

Livello qualitativo

Caratteristiche

endcapped

Produttore/marchio commerciale

SUPELCOSIL

Grado di funzionalizzazione

6.0% carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable

Lungh. × D.I.

15 cm × 4.6 mm

Area superficiale

170 m2/g

Copertura della superficie

surface coverage 3.2 μmol/m2

Matrice

silica gel, spherical particle platform

Gruppo funzionale matrice

C8 (octyl) phase

Dimensione particelle

3 μm

Dimensione pori

120 Å

Intervallo di pH

2-7.5

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.

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Note legali

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
Thomas Borch et al.
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
S B Ruddy et al.
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium

Protocolli

HPLC Analysis of Free Fatty Acids on SUPELCOSIL™ LC-8

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