MABS2219

Anti-MGAT1 Antibody, clone 5F5

• Sinonimo/i: Anti-MGAT1 Antibody, clone 5F5
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 5F5, monoclonal
• PM: calculated mol wt 38.79 kDa; observed mol wt ~36 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: mouse
• Confezionamento: antibody small pack of 100 μg
• tecniche: western blot: suitable
• Isotipo: IgG2κ
• Sequenza dell’epitopo: C-terminal half
• N° accesso ID proteina: NP_080989
• N° accesso UniProt: Q91ZV4
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Mogat1(68393)

Descrizione

Descrizione generale

2-acylglycerol O-acyltransferase 1 (UniProt: EC:2.3.1.22, Acyl-CoA:monoacylglycerol acyltransferase 1, MGAT1,Diacylglycerol acyltransferase 2-like protein 1, Monoacylglycerol O-acyltransferase 1, MGAT1) is encoded by the Moghat1 (also known as Dgat2l1) gene (Gene ID: 68393) in murine species. MGAT1 is an enzyme that catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Its activity is reported to be directly regulated by PPARg and it plays a role in regulating the pathway that leads to incorporation of fatty acids into triglycerides. MGAT1 activity is shown to be higher in mice fed high fat diet and by overexpression of PPARg, which leads to hepatic steatosis. Even in cultured hepatocytes, overexpression of MGAT1 is shown to increase triglyceride synthesis without affecting the expression of PPARg. The binding of PPARg to MGAT1 promoter is significantly enhanced Ob/Ob and high fat diet fed mice. MGAT1 expression is significantly reduced in PPARg knockdown murine models and knockdown of MGAT1 expression is shown to improve hepatic steatosis in high fat fed mice. It has also been shown that C3H mice that do not express PPARg and MGAT1 in the liver do not develop hepatic steatosis after being fed a high fat diet. (Ref.: Lee, YJ., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34); 13656-13661).

Specificità

Clone 5F5 is a mouse monoclonal antibody that detects Monoacylglycerol O-acyltransferase 1 (MGAT1). It targets an epitope within 13 amino acids from the C-terminal half.

Immunogeno

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of murine 2-acylglycerol O-acyltransferase 1 (MGAT1).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293 cells overexpressing MGAT1.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected MGAT1 in lysates from HEK293 cells transfected with mouse MGAT1, but not in untransfected cells.

Tested Applications

Western Blotting Analysis: A representative lot detected MGAT1 in Western Blotting applications (Lee, Y.J., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(34):13656-61).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MGAT1, clone 5F5, Cat. No. MABS2219, is a mouse monoclonal antibody that detects 2-acylglycerol O-acyltransferase 1 (MGAT1) and is tested for use in Western Blotting,

Stato fisico

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable