MABS1812M

Anti-Furin Antibody, clone 16B1.1

clone 16B1.1, from mouse

• Sinonimo/i: Anti-FUR, Anti-PACE, Anti-PCSK3, Anti-SPC1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 16B1.1, monoclonal
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25 μg
• tecniche: western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001276752
• N° accesso UniProt: P09958
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... FURIN(5045)

Descrizione

Descrizione generale

Furin (UniProt: P09958; also known as EC: 3.4.2.75, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE) is encoded by the FURIN (also known as FUR, PACE, PCSK3) gene (Gene ID: 5045) in human. Furin is an ubiquitously expressed enzyme with endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. It is single-pass type I membrane protein that shuttles between the trans-Golgi network and the cell surface. It is synthesized with a signal peptide of 26 amino acids and a propeptide region (aa 27-107) that inhibits its activity. The inhibition peptide plays the role of an intramolecular chaperone. It is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Propeptide cleavage is a prerequisite for exit of Furin molecules out of the endoplasmic reticulum. A second cleavage within the propeptide region occurs in the trans-Golgi network, followed by the release of the propeptide and the activation of Furin. Mature Furin has a luminal region (aa 108-705), a helical domain (aa 716-738), and a cytoplasmic region (aa 739-794). The cytoplasmic domain responsible for its trans-Golgi network localization and recycling from the cell surface.

Specificità

Clone 16B1.1 detects human Furin and it targets an epitope within 177 amino acids from the C-terminal half.

Immunogeno

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Furin.

Applicazioni

Anti-Furin, clone 16B1.1, Cat. No. MABS1812, is a mouse monoclonal antibody that detects Furin in human cells and has been tested for use in Western Blotting.

Qualità

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Furin in 10 µg of U-251 cell lysate.

Descrizione del bersaglio

~100 kDa observed; 86.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1