MABN2526

Anti-Aquaporin 4 Antibody

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: E5415B, monoclonal
• PM: calculated mol wt 34.44 kDa; observed mol wt ~45 kDa
• Reattività contro le specie: mouse
• Confezionamento: antibody small pack of 100 μg
• tecniche: ELISA: suitable; activity assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso UniProt: P55088
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: -10 to -25°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Aqp4(11829)

Descrizione

Descrizione generale

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis Optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).

Specificità

Clone E5415B is a mouse monoclonal antibody generated in AQP-4 knockout mice that detects murine Aquaporin-4. It targets an epitope within the extracellular domain. This antibody is specific for M23 (Isoform 1).

Immunogeno

Recombinant extracellular domain of murine Aquaporin 4.

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in Mouse heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Aquaporin 4 in Mouse heart tissue lysate.

Tested Applications

ELISA Analysis: A representative lot detected Aquaporin 4 in ELISA applications (Huang, P, et. al. (2016). Biochem Biophys Rep. 7:77-83).

Western Blotting Analysis: A representative lot detected Aquaporin 4 in Western Blotting applications (Huang, P, et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Aquaporin 4 in mouse astrocytes.

Function Analysis: A representative lot reduced endogenous Aquaporin-4 in primary cultured astrocytes. (Huang, P, et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunofluorescence Analysis: A representative lot detected Aquaporin 4 in Immunofluorescence applications (Huang, P, et. al. (2016). Biochem Biophys Rep. 7:77-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Aquaporin 4, clone E5415B, Cat. No. MABN2526, is a mouse monoclonal antibody that detects Aquaporin-4 and is tested for use in ELISA, Function Analysis, Immunocytochemistry, Immunofluorescence, and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stoccaggio e stabilità

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable