MABN1838

Anti-AGE Antibody, Methylglyoxal Antibody, clone 3D11

clone 3D11, from mouse

• Sinonimo/i: Methylglyoxal, AGE Product methylglyoxal, clone 3D11
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 3D11, monoclonal
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): human, rat
• tecniche: ELISA: suitable; immunohistochemistry: suitable (paraffin)
• Isotipo: IgG2aλ
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Chronic accumulation of Advanced Glycation End products (AGEs) is a common feature during aging, hyperlipidemia, and diabetes and affects function of proteins, lipids, and DNA. Although formation of AGE products is considered to be predominantly an endogenous process, certain external factors, such as some dietary products and smoking can increase the level of AGE in tissues. Under certain conditions glucose may form covalent adducts with the plasma proteins through the process of glycation. Glycation is a non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of proteins. The term AGE often refers to non-reactive terminal products, such as N- carboxymethyllysine (CML) as well as reactive precursors, such as methylglyoxal (MG), which is generated through a non-oxidative process from triose phosphates during anaerobic glycolysis. MG reacts with arginine, lysine, and cysteine residues in proteins to form AGE products. Excessive glycation can disrupt protein function by altering enzyme activity, receptor function, and molecular conformation. Protein glycation and subsequent formation of AGE products contributes to the pathogenesis of diabetes-related complications, such as retinopathy, nephropathy, and neuropathy. AGE products interact with a variety of cell-surface AGE-binding receptors (RAGE) that results in their endocytosis and degradation. It can also generate pro-oxidant and pro-inflammatory events in cells. AGE products could also contribute to the development or worsening of many degenerative diseases, including Alzheimer′s disease. (Ref.: Cai,W., et al. (2002). Mol. Med 8 (7): 337-346).

Specificità

Clone 3D11 specifically reacts with methylglyoxal modified proteins. It does not react with CML, CEL, or other AGE epitopes.

Immunogeno

Methylglyoxal-cojugated Ovalbumin.

Applicazioni

Anti-AGE, Methylglyoxal, clone 3D11, Cat. No. MABN1838, is a highly specific mouse monoclonal antibody that targets Methylglyoxal modified proteins and has been tested in ELISA and Immunohistochemistry (Paraffin).

ELISA Analysis: A representative lot detected AGE, Methylglyoxal in ELISA applications (Vlassara, H., et. al. (2002). Proc Natl Acad Sci USA. 99(24):15596-601; Cai, W., et. al. (2008). Am J Pathol. 173(2):327-36; Cai, W., et. al. (2012). Proc Natl Acad Sci USA. 109(39):15888-15893).

Research Category
Neuroscience

Qualità

Evaluated by Immunohistochemistry in mouse kidney tissue.

Immunohistochemistry Analysis: A 1:3,000 dilution of this antibody detected AGE, Methylglyoxal in kidney tissue from mice fed high AGE diets.

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1