MABF981

Anti-CXCR4 Antibody, clone 12G5

clone 12G5, from mouse

• Sinonimo/i: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, HM89, LCR1, Leukocyte-derived seven transmembrane domain receptor, LESTR, Lipopolysaccharide-associated protein 3, LAP-3, LPS-associated protein 3, NPYRL, Stromal cell-derived factor 1 receptor, SDF-
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 12G5, monoclonal
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; neutralization: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001008540
• N° accesso UniProt: P61073
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CXCR4(7852)

Descrizione

Descrizione generale

C-X-C chemokine receptor type 4 (UniProt P61073; also known as CD184, CXC-R4, CXCR-4, FB22, Fusin, HM89, LAP-3, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, Lipopolysaccharide-associated protein 3, LPS-associated protein 3, Neuropeptide Y receptor Y3, NPYRL, SDF-1 receptor, Stromal cell-derived factor 1 receptor) is encoded by the CXCR4 (also known as D2S201E, HSY3RR, LAP3, NPY3R, NPYR, NPYY3R, WHIM, WHIMS) gene (Gene ID 7592) in human. Chemokines and their receptors direct migration of distinct leukocyte subsets to sites of inflammation and to their specific niches in lymphoid organs. Virtually all T cell chemoattractants selectively attract memory/activated T cells. CXCR4 is the seven-transmembrane receptor for SDF-1 that is essential for the development of non-hematopoietic tissues, including heart and B lymphocytes. CXCR4 and CCR5 are two chemokine receptors that also function as co-receptors for human immunodeficiency virus (HIV) entry into CD4+ cells. During the early stages of HIV infection, viral isolates tend to use CCR5 for viral entry, while later isolates tend to use CXCR4.

Immunogeno

Epitope: Extracellular domain

Simian immunodeficiency virus (SIVmac) variant CP-MAC-infected Sup-T1 human T lymphoblasts.

Applicazioni

Flow Cytometry Analysis: A representative lot detected surface expression of human CXCR4 in various cell types by flow cytometry (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930; McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696; Endres, M.J., et al. (1996). Cell. 87(4):745-756)
Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed CHO cells transfected to stably express human CXCR4/fusin (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Neutralizing Analysis: A representative lot blocked SDF-1-induced lymphocyte chemotaxis using freshly isolated human PBMCs (Bleul, C.C., et al. (1997). Proc. Natl. Acad. Sci. U S A.94(5):1925-1930).
Neutralizing Analysis: A representative lot blocked the infection of CXCR4+/CD4+ rhabdomyosarcoma (RD) cells by seven HIV-1 (LAI, RF, Gun-1wt, Gun-1var) and HIV-2 (ROD A/B and CBL-23) strains as assessed by >95% reduced syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion. Clone 12G5 also blocked Gun-1wt, Gun-1var, and CBL-23, but not the other 4 HIV strains from infecting HeLa/CD4 and C8166 cultures (McKnight, A., et al. (1997). J. Virol. 71(2):1692-1696).
Neutralizing Analysis: A representative lot blocked HIV-2 /vcp strain from infecting U87 cells transfected to express human CXCR4/fusin as assessed a reduction of syncytium (multinucleated cell) formation due to viral infection-induced cell-to-cell fusion (Endres, M.J., et al. (1996). Cell. 87(4):745-756).
Note: Although clone 12G5 binds cell surface CXCR4, efforts to immunoprecipitate or immunoblot CXCR4 were reported to be unsuccessful (Endres, M.J., et al. (1996). Cell. 87(4):745-756).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-CXCR4 Antibody, clone 12G5 is validated for use in Flow Cytometry, Immunocytochemistry and Neutralizing for the detection of CXCR4.

Qualità

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CXCR4 in Jurkat cells.

Descrizione del bersaglio

~39 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable