MABF2791

Anti-CCR9 Antibody, clone 7E7

• Sinonimo/i: C-C chemokine receptor type 9;C-C CKR-9;CC-CKR-9;CCR-9;Chemokine C-C receptor 10;CDw199
• Codice UNSPSC: 12352203
• NACRES: NA.43
• Clone: 7E7, monoclonal
• application: FACS; IF; IHC (p)
• Reattività contro le specie: mouse, human
• tecniche: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Proprietà

• Origine biologica: rat
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 7E7, monoclonal
• PM: calculated mol wt 41.91 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: mouse, human
• Confezionamento: antibody small pack of 100 μg
• tecniche: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotipo: IgG2b
• Sequenza dell’epitopo: N-terminal
• N° accesso ID proteina: NP_001160097
• N° accesso UniProt: Q9WUT7
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TACSTD2(4070)

Descrizione

Descrizione generale

C-C chemokine receptor type 9 (UniProt: Q9WUT7; also known as C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10, CDw199) is encoded by the Crr9 (also known as Cmkbr10) gene (Gene ID: 12769) in murine species. CCR9 is a multi-pass membrane protein of the G-protein coupled receptor 1 family. It has four extracellular domains, seven transmembrane domains, and four cytoplasmic domains and serves as a receptor for chemokine CCL25/TECK. It is highly expressed in the thymus tissue. It is also expressed on virtually all small intestinal T cells and IgA-producing plasma cells (PCs) from the spleen. It is reported to be crucial for the positioning of PCs to the small intestine. Higher expression of CCR9 is observed in newly generated PCs and it is down-regulated once these cells reach their final destination in the intestine. In wild-type mice, a majority of the PCs are shown to locate to the lower half of the villus where CCL25 is also expressed at high levels. CCR9 deficient mice exhibit reduced number of PCs in the intestine and are unable to mount a T cell dependent antigen specific IgA response. CCR9-/- hematopoietic stem cells are deficient in their ability to generate all major thymocyte subsets. (Ref.: Svensson, M., et al. (2008). J. Leukoc. Biol. 83(1); 156-164; Pabst, O., et al. (2004). J. Exp. Med. 199(3); 411-416).

Specificità

Clone 7E7 is a rat monoclonal antibody that detects C-C chemokine receptor type 9 (CCR9). It targets an epitope within 20 amino acids from the fist extracellular domain in the N-terminal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the first extracellular domain in the N-terminal region of mouse C-C-chemokine receptor type 9 (CCR9).

Applicazioni

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse thymocytes.

Flow Cytometry Analysis: 1 μg of this antibody detected CCR9 in one million BALB/c mouse thymocytes.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CCR9 in Immunohistochemistry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Flow Cytometry Analysis: A representative lot detected CCR9 in Flow Cytometry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15; Svensson, M., et al. (2008). J Leukoc Biol. 83(1):156-64; Chennupati, V., et al. (2010). J Immunol. 185(9):5160-8).

Immunofluorescence Analysis: A representative lot detected CCR9 in Immunofluorescence applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CCR9, clone 7E7, Cat. No. MABF2791, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.

Stato fisico

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1