MABE915

Anti-Mi-2β Antibody, clone 2G8

clone 2G8, from mouse

• Sinonimo/i: Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, Mi-2b
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 2G8, monoclonal
• application: ChIP; WB
• Reattività contro le specie: human
• tecniche: ChIP: suitable (ChIP-seq); western blot: suitable
• citations: 1

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 2G8, monoclonal
• Reattività contro le specie: human
• tecniche: ChIP: suitable (ChIP-seq); western blot: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001264
• N° accesso UniProt: Q14839
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CHD4(1108)

Descrizione

Descrizione generale

Chromodomain-helicase-DNA-binding protein 4 (CHD-4), often referred to as Mi-2β, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. Mi-2β has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of Mi-2β.

Applicazioni

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP (see Zhang, J., et al 2012).

ChIP-seq: A representative lot of this antibody used as part of an antibody cocktail of consisting of Anti-Mi-2β clones 2G8, 16G4 and 17H11 (cat. no. MABE915, MABE914, and MABE920 respectively) was reported to work in ChIP-seq (see Zhang, J., et al 2012).

This Anti-Mi-2β Antibody, clone 2G8 is validated for use in Western Blotting and ChIP-seq and Culture Confirmation for the detection of Mi-2β.

Qualità

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in 10 µg of a Jurkat cell lysate.

Descrizione del bersaglio

~ 220 kDa observed

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable