MABE344

Anti-BICC1/Protein Bicaudal C Homolog 1 Antibody, clone 2D9.1

clone 2D9.1, from mouse

• Sinonimo/i: Protein bicaudal C homolog 1, Bic-C
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 2D9.1, monoclonal
• Reattività contro le specie: human, mouse
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001073981
• N° accesso UniProt: Q9H694
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... BICC1(80114)

Descrizione

Descrizione generale

Protein bicaudal C homolog 1 (UniProt Q9H694; also known as Bic-C) is encoded by the BICC1 (also known as CYSRD) gene (Gene ID 80114) in human. Drosophila bicaudal C and its mammalian homologs are RNA-binding proteins involved in symmetric distribution and localized translation of mRNAs of many proteins in a wide range of cells and tissues. RNA binding of Bic-C is mediated by N-terminal KH domains (a.a. 132-199 & 284-348), whereas a C-terminal sterile alpha motif (SAM; a.a. 873-936) self-polymerizes and localizes Bic-C at cytoplasmic foci where silencing of bound mRNAs takes place. Defective polymerization results in decreased Bic-C stability and thereby attenuates inhibition of Dishevelled-2 of Wnt/β-catenin pathway. Loss of BICC1 provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning due to defective silencing of Bic-C target mRNAs, including adenylate cyclase 6 (AC6).

Specificità

Clone 2D9.1 reacts with both spliced isoforms of human and murine BICC1 reported by UniProt (Q9H694 & Q99MQ1).

Immunogeno

Epitope: N-terminal half.

Recombinant fragment corresponding to the N-terminal half of human BICC1/Protein Bicaudal C Homolog 1.

Applicazioni

Anti-BICC1 Antibody, clone 2D9.1 is an antibody against Protein Bicaudal C Homolog 1 for use in Western Blotting, Immunoprecipitation.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blotting Analysis: 1 µg/mL from a representative lot detected Bic-C/BICC1 in human HK and murine mIMCD-3 kidney cell lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY)
Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY).

Qualità

Evaluated by Western Blotting of recombinant human BICC1.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1.

Descrizione del bersaglio

~130 kDa observed. 104.8/105.0 kDa (Human/mouse isoform 1) and 94.29/93.41 kDa (Human/mouse isoform 2) calculated.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable