MABC593

Anti-Astrin/SPAG5, clone 8B2 Antibody

clone 8B2, from rat

• Sinonimo/i: Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 8B2, monoclonal
• Reattività contro le specie: human
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2cκ
• N° accesso NCBI: NP_006452
• N° accesso UniProt: Q96R06
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SPAG5(10615)

Descrizione

Descrizione generale

Astrin/SPAG5 is a microtubule binding and cytoskeleton associated signaling protein that plays not only a critical role in mitotic spindle and chromosome segregation but also as an important signal transducer in mTOR and GSK3 beta signaling pathways. Astrin/SPAG5 is localized in the cytoplasm and/or mitotic apparatus (in dividing cells) and centrosomes. In non-dividing cells Astrin/SPAG5 is associated with microtubules unless under cellular stress, then it is localized in stress granules. Astrin/SPAG5 is expressed in many tissues but is very high in testis and sperm and is regulated via the cell cycle highest levels are seen in prophase. Recent research has focused on Astrin/SPAG5 critical role in regulating mTORC1 and its effects on growth and survival. When cells are stress Astrin/SPAG5 inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules (SGs), thereby preventing mTORC1-hyperactivation-induced apoptosis. Because cancerous tumor cells are often stressed, this mechanism is believed to be key to their survival, thus Astrin/SPAG5 activity repression is being examined as potential anti-cancer therapy.

Immunogeno

Linear peptide corresponding to human Astrin/SPAG5.

Applicazioni

Immunocytochemistry Analysis: A representative lot detected Astrin/SPAG5 HeLa cells (Thedieck, K., et al. (2013). Cell. 154:859-874).
Western Blotting Analysis: A representative lot detected Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Astrin/SPAG5, clone 8B2 Antibody is validated for use in Immunocytochemistry and Western Blotting and Immunoprecipitation for the detection of Astrin/SPAG5.

Qualità

Evaluated by Immunocytochemistry in HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Astrin/SPAG5 in HeLa and A431 cells.

Descrizione del bersaglio

134 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable