MABC32

Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2

clone 11C9.2, from mouse

• Sinonimo/i: sequestosome 1, EBI3-associated protein of 60 kDa, Paget disease of bone 3, phosphotyrosine independent ligand for the Lck SH2 domain p62, oxidative stress induced like, EBI3-associated protein p60, Phosphotyrosine-independent ligand for the Lck SH2 doma
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 11C9.2, monoclonal
• Reattività contro le specie: mouse, rat, human
• tecniche: flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgMκ
• N° accesso NCBI: NP_001135770
• N° accesso UniProt: Q13501
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SQSTM1(8878)

Descrizione

Descrizione generale

p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis.

Immunogeno

GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1).

Applicazioni

Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.

Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.

Qualità

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.

Descrizione del bersaglio

~47-60 kDa observed.
The calculated molecular weight of this protein is 47 kDa and also has an isoform at 38 kDa Due to modifications, this protein may be observed up to ~60 kDa in some lysates.

Stato fisico

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
A431 cell lysate

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable