MABC166
Anti-Angiogenin Antibody, clone 26–2F (Azide Free)
clone 26-2F, from mouse
Sinonimo/i:
Angiogenin, Ribonuclease 5, RNase 5
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
26-2F, monoclonal
Reattività contro le specie
human
tecniche
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... ANG(283)
Descrizione generale
Angiogenin (Ang) is a non-glycosylated polypeptide, 123 amino acids in length, with a molecular mass of 14 kDa. It is secreted by tumor cells and is a potent inducer of neovascularization. Angiogenin is produced by a variety of cell types including vascular endothelial cells and smooth muscle cells, fibroblasts, tumor colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. It specifically binds to endothelial cells and elicits second messenger systems. Angiogenin is present in human follicular fluid, and its production is up-regulated by human chorionic gonadotropin and hypoxic conditions. Angiogenin shows a high degree of homology with known ribonucleases such as pancreatic ribonuclease A, and the capacity of angiogenin to induce blood vessel growth is dependent on its ribo-nucleolytic activity. Angiogenin is thought to be involved in the development of solid tumors; angiogenin antagonists are capable of inhibiting tumor growth. Angiogenin is endocytosed by subconfluent endothelial cells and translocated to the nucleus, where it accumulates in the nucleolus. Serum angiogenin concentration is increased in serum of colorectal cancer patients, and correlates with cancer progression. The cytoskeletal protein α-actinin-2 binds to angiogenin, which might be a novel interface target for anti-angiogenesis and anti-tumor therapy.
Immunogeno
Purified Human Angiogenin.
Applicazioni
Inhibition Assay: A representative lot of this antibody blocked cell-free protein synthesis by angiogenin (Fett et al., (1994) Biochemistry. 33(18):5421-5427).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Angiogenin Antibody, clone 26–2F (Azide Free) is validated for use in Immunocytochemistry and Inhibition and Immunohistochemistry for the detection of Angiogenin.
Qualità
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Descrizione del bersaglio
16 kDa calculated
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Chimeric anti-angiogenin antibody cAb 26-2F inhibits the formation of human breast cancer xenografts in athymic mice.
Piccoli, R, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 4579-4583 (1998)
Takanori Tsuji et al.
Cancer research, 65(4), 1352-1360 (2005-03-01)
Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is
A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angiogenic activities and localization of the antigenic epitope.
Fett, J W, et al.
Biochemistry, 33, 5421-5427 (1994)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.