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MAB8419

Sigma-Aldrich

Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227

clone 227, Chemicon®, from mouse

Sinonimo/i:

Trans-Activator X Gene Product

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

227, monoclonal

Reattività contro le specie

human

Produttore/marchio commerciale

Chemicon®

tecniche

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

Condizioni di spedizione

wet ice

Descrizione generale

Protein X (UniProt: P69713; also known as HBx, Peptide X, pX) is encoded by the X gene (Gene ID: 944566) in Hepatitis B virus. Hepatitis B virus (HBV) contains seven main proteins, which include Core, pre-Core, Small S, Middle S, Large S, Polymerase, and the HBx protein. HBx, a multifunctional protein, although not essential for HBV infection, plays a role in silencing host antiviral defenses and promoting viral transcription. It facilitates the efficient replication of HBV by stimulating HBV gene expression from the cccDNA template. It is mainly localized in the cytoplasm with a small fraction detected in the nucleus. However, its expression level can also influence its cellular localization. It is predominantly nuclear when expressed in cells at very low levels but becomes largely cytoplasmic as its expression level increases. In cytoplasm, a minor fraction may also associate with mitochondria. Its mitochondrial targeting sequence is localized in amino acids 68-117. HBx has an N-terminal negative regulatory domain and its transactivation or coactivation of the C-terminal domain is reported to interfere with host cell signaling transduction pathways to help HBV replication. The best-characterized HBx binding partner is the cellular damage-specific DNA binding protein 1 (DDB1) and the interaction of HBx-DDB1 is essential for HBV replication. HBx promotes the degradation of the cellular structural maintenance of chromosomes 5/6 complex (Smc5/6), which directly binds DNA and entraps DNA plasmids. HBx is directly involved in development of cirrhosis and liver and hepatocellular carcinoma. (Ref.: Kornyeyev, D., et al. (2019). J. Virol. 93(16); e00248-19; Hwang, G-Y., et al. (2003). J. Clin. Microbiol. 41(12); 5598-5603).

Specificità

Reacts with Hbx-protein (trans-activator X gene product) of hepatitis B virus (epitope recognized - amino acid positions 90-115).

SPECIES REACTIVITIES:
Reacts only with HBV-infected human cells.

Immunogeno

Epitope: X-Protein, aa 90-115
Peptide cooresponding to aa90-115 of h HBV-X protein.

Applicazioni

Anti-Hepatitis B Virus Antibody, X-Protein, a.a. 90-115, clone 227 detects level of Hepatitis B Virus & has been published & validated for use in ELISA, WB & IC.
ELISA, immunoblotting and immunocytochemistry staining of infected liver tissues and HBV-transfected hepatoma cells. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Stato fisico

Format: Purified
Liquid in 0.02M PBS pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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HBX causes cyclin D1 overexpression and development of breast cancer in transgenic animals that are heterozygous for p53.
Klein, A; Guhl, E; Tzeng, YJ; Fuhrhop, J; Levrero, M; Graessmann, M; Graessmann, A
Oncogene null
Accumulation of 8-hydroxy-2'-deoxyguanosine adducts in HBx recombinant HepG2 cells and HBx transgenic mice.
Ralph Gehrke, Maria A Brauchle, Kurt Reifenberg, Eberhard Hildt, Uwe Gruetzner et al.
Digestion null
Epigenetic modification induced by hepatitis B virus X protein via interaction with de novo DNA methyltransferase DNMT3A.
Da-Li Zheng, Li Zhang, Na Cheng, Xiao Xu, Qing Deng, Xiao-Mei Teng, Ke-Sheng Wang et al.
Journal of Hepatology null
T M Bui-Nguyen et al.
Oncogene, 29(8), 1179-1189 (2009-12-17)
Metastasis-associated protein 1 (MTA1), a master chromatin modifier, has been shown to regulate cancer progression and is widely upregulated in human cancer, including hepatitis B virus-associated hepatocellular carcinomas (HCCs). Here we provide evidence that hepatitis B virus transactivator protein HBx
KyeongJin Kim et al.
PloS one, 5(3), e8649-e8649 (2010-03-31)
Hepatitis B virus (HBV) is a major cause of chronic liver diseases, and frequently results in hepatitis, cirrhosis, and ultimately hepatocellular carcinoma. The role of HCV in associations with insulin signaling has been elucidated. However, the pathogenesis of HBV-associated insulin

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