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Key Documents

MAB5256A5

Sigma-Aldrich

Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor™ 555 Conjugate

clone NE14, from mouse, ALEXA FLUOR™ 555

Sinonimo/i:

Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Coniugato

ALEXA FLUOR™ 555

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

NE14, monoclonal

Reattività contro le specie

mouse, rat, pig

Reattività contro le specie (prevista in base all’omologia)

human (100% sequence homology), porcine (immunogen homology)

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable

Isotipo

IgG1

N° accesso NCBI

NP_066554

N° accesso UniProt

P12036

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Descrizione generale

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information and epitope maps, search "neurofilament" on our website.

Applicazioni

Detect Neurofilament 200kDa using this Anti-Neurofilament 200kDa Antibody, clone NE14, Alexa Fluor 555 Conjugate validated for use in ICC & IHC.

Qualità

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neurofilament in rat E18 primary cortex cells.

Altre note

Unconjugated Anti-Neurofilament has been shown to work with multiple species including Human and Porcine.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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