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Merck
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Key Documents

MAB4419

Sigma-Aldrich

Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2

clone 7F9.2, from mouse

Sinonimo/i:

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

7F9.2, monoclonal

Reattività contro le specie

human, mouse

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

input

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

Isotipo

IgG1κ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... POU5F1(5460)
mouse ... Pou5F1(18999)

Descrizione generale

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Specificità

Reactivity to other species has not been determined.
This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform.

Immunogeno

Recombinant human Oct-4 protein, a.a. 1-145.

Applicazioni

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1].
Western Blotting: Recommended working dilution is 1 - 2 µg/mL.

Flow Cytometry: Recommended working dilution is 2 µg/mL.

Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200.

Descrizione del bersaglio

~39 kDa

Linkage

Replaces: MAB4305

Stato fisico

Format: Purified
Protein G sepharose column
Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3.

Stoccaggio e stabilità

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Gene targeting and subsequent site-specific transgenesis at the ?-actin (ACTB) locus in common marmoset embryonic stem cells.
Seiji Shiozawa,Kenji Kawai,Yohei Okada,Ikuo Tomioka,Takuji Maeda,Akifumi Kanda et al.
Stem Cells and Development null
Gisele C M Dias et al.
Molecular reproduction and development (2020-06-20)
The objective of this study was to establish a protocol for the characterization, isolation, and culture of type A spermatogonia using specific molecular markers for these cells in fish. To this end, adult Prochilodus lineatus testes were collected and digested
Pushpa Singh et al.
Stem cell research & therapy, 13(1), 60-60 (2022-02-07)
True identity and specific set of markers to enrich endometrial stem cells still remains elusive. Present study was undertaken to further substantiate that very small embryonic-like stem cells (VSELs) are the true and elusive stem cells in adult mice endometrium.
Teresa Starzyńska et al.
Journal of cellular and molecular medicine, 17(6), 792-799 (2013-05-16)
Various experimental studies indicate potential involvement of bone marrow (BM)-derived stem cells (SCs) in malignancy development and progression. In this study, we comprehensively analysed systemic trafficking of various populations of BM-derived SCs (BMSCs), i.e., mesenchymal, haematopoietic, endothelial stem/progenitor cells (MSCs
Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus).
Lin, ZY; Imamura, M; Sano, C; Nakajima, R; Suzuki, T; Yamadera, R; Takehara, Y; Okano et al.
Reproduction (Cambridge, England) null

Articoli

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The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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