MAB4401

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon^®, from mouse

• Sinonimo/i: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Forma dell’anticorpo: purified antibody
• Clone: 10H11.2, monoclonal
• Reattività contro le specie: human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• input: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• Isotipo: IgG1
• N° accesso NCBI: NM_002701.4
• N° accesso UniProt: Q01860
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POU5F1(5460)

Descrizione

Descrizione generale

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Purified monoclonal antibody for the detection of Human Oct-4.

Specificità

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).

The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

Applicazioni

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.

Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

Qualità

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

Descrizione del bersaglio

44 kDa

Stato fisico

Format: Purified

Risultati analitici

Control
Human embryonic stem cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°C): Not applicable