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Documenti fondamentali

MAB349

Sigma-Aldrich

Anti-Beta (β)-Amyloid antibody

CHEMICON®, mouse monoclonal, 1.D5

Sinonimo/i:

Alz-90

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nome del prodotto

Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5, clone 1.D5, Chemicon®, from mouse

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

1.D5, monoclonal

Reattività contro le specie

human, rat

Produttore/marchio commerciale

Chemicon®

tecniche

western blot: suitable

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... APP(351)

Specificità

Reacts with the natural amyloid precursor protein A4(695).

Immunogeno

Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].

Applicazioni

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Western blot: 10-20 μg/mL

Optimal working dilutions must be determined by the end user.

Westen Blot Protocol for MAB349

1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.

2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.

3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.

4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.

5) Repeat step #3.

6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.

7) Repeat step #3.

8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.

9) Repeat step #3.

Stato fisico

Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Stoccaggio e stabilità

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.
Maya Tanaka,Tatsuya Abe,Yoshitaka Hara
Journal of Cellular Physiology null
Axonal injury in young pediatric head trauma: a comparison study of ?-amyloid precursor protein (?-APP) immunohistochemical staining in traumatic and nontraumatic deaths.
Johnson, MW; Stoll, L; Rubio, A; Troncoso, J; Pletnikova, O; Fowler, DR; Li, L
Journal of Forensic Sciences null
Magdolna Pákáski et al.
Neurochemistry international, 47(3), 190-195 (2005-06-16)
Comorbid depression of Alzheimer's disease (AD) is a common mood disorder in the elderly and a broad spectrum of antidepressants have been used for its treatment. Abeta peptides and other derivatives of the amyloid precursor protein (APP) have been implicated
Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid protein.
Weidemann, A, et al.
Cell, 57, 115-126 (1989)
3,4-Methylenedioxymethamphetamine (MDMA), but not morphine, alters APP processing in the rat brain.
Kalman, Janos, et al.
The International Journal of Neuropsychopharmacology, 10, 183-190 (2007)

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