MAB331-C

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free)

clone SP14, from mouse

• Sinonimo/i: Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP-25
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: SP14, monoclonal
• Reattività contro le specie: monkey, bovine, mouse, rat, guinea pig, human
• tecniche: activity assay: suitable; electron microscopy: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_570824
• N° accesso UniProt: P60880
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SNAP25(6616)

Descrizione

Descrizione generale

Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.

Specificità

Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.

Immunogeno

Epitope: N-terminal half

Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

Applicazioni

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualità

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.

Descrizione del bersaglio

~25 kDa observed

Linkage

Replaces: MAB331

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable