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Key Documents

MAB3077

Sigma-Aldrich

Anti-G Protein Giα-2 Antibody, clone L5

clone L5, Chemicon®, from mouse

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

L5, monoclonal

Reattività contro le specie

bovine, mouse, human, rat, guinea pig

Produttore/marchio commerciale

Chemicon®

tecniche

western blot: suitable

Isotipo

IgG2b

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... GNAI2(2771)

Specificità

Gialpha-2. Strong reactivity with bovine (100%), mouse (69%), rat (69%), human (62%) and guinea pig (43%). Specificity confirmed by Western blot versus bovine Galphat, rat Goalpha, rat Gialpha-1, Gialpha-2, rat Gialpha-3, bovine Gsalpha, and murine GoalphaA and GoalphaB.

Immunogeno

Recombinant Gialpha-2, purified by the method of Linder (1990).

Applicazioni

Anti-G Protein Giα-2 Antibody, clone L5 is an antibody against G Protein Giα-2 for use in WB.
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:500 - 1:1000, with detection by 125I-labeled anti-IgG.

Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.

Optimal working dilutions must be determined by end user.

Stato fisico

Format: Purified
Purified immunoglobulin, in 0.02M phosphate buffer, 0.25M sodium chloride, pH 7.6, with 0.1% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Chronic but not acute intracerebroventricular administration of amyloid beta-peptide(25-35) decreases somatostatin content, adenylate cyclase activity, somatostatin-induced inhibition of adenylate cyclase activity, and adenylate cyclase I levels in the rat hippocampus.
E Burgos-Ramos,A Hervas-Aguilar,L Puebla-Jimenez,M C Boyano-Adanez,E Arilla-Ferreiro
Journal of Neuroscience Research null
Hiromi Ohara et al.
The Journal of veterinary medical science, 75(1), 85-88 (2012-08-11)
In rodents, Gα(i2)-expressing sensory neurons (SNs) that co-express vomeronasal receptor type 1 (V1R) are specifically found in the vomeronasal organ (VNO) and project their axons to the accessory olfactory bulb (AOB). In goats, however, Gα(i2)/V1R-expressing SNs exist in both the
Kati S Mönkkönen et al.
Human reproduction (Oxford, England), 22(5), 1224-1230 (2007-03-10)
Heterotrimeric G proteins take part in membrane-mediated cell signalling and have a role in hormonal regulation. This study clarifies the expression and localization of the G protein subunit G alpha(i2) in the human endometrium and Fallopian tube and changes in
J T O'Flaherty et al.
Journal of immunology (Baltimore, Md. : 1950), 164(6), 3345-3352 (2000-03-08)
5-Oxo-eicosatetraenoic acid (5-oxoETE) stimulated human neutrophil (PMN) and eosinophil chemotaxis, PMN hexose uptake, and PMN membrane GTP/GDP exchange. Pertussis toxin (PT), a blocker of heterotrimeric G proteins (GP), completely inhibited these responses, but proved far less effective on the same
Activity of the hippocampal somatostatinergic system following daily administration of melatonin.
Rosa Maria Izquierdo-Claros, Maria Del Carmen Boyano-Adanez, Eduardo Arilla-Ferreiro
Brain Research Molecular Brain Research null

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