MAB1397Z

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

clone 3E2B, Chemicon^®, from hamster(Armenian)

• Sinonimo/i: CD54, MAB1397
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: hamster (Armenian)
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 3E2B, monoclonal
• Reattività contro le specie: mouse
• Produttore/marchio commerciale: Chemicon^®
• tecniche: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• Isotipo: IgG
• N° accesso NCBI: NM_000201.1
• N° accesso UniProt: P05362
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Icam1(15894)

Descrizione

Specificità

MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.

Applicazioni

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.

Immunocytochemistry: Live or acetone fixed cells

ELISA

Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

Clone 3E2B is reported to be function blocking against murine ICAM-1.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Linkage

Replaces: CBL1331F

Stato fisico

Format: Purified

Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg

Stoccaggio e stabilità

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable