GR15

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

liquid, clone EGFR.1, Calbiochem^®

• Sinonimo/i: Anti-Epidermal Growth Factor Receptor
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: EGFR.1, monoclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human
• Non deve reagire con: rat, mouse
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: do not freeze
• Isotipo: IgG2b
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Descrizione

Descrizione generale

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

Immunogeno

Human

partially purified, human EGFR from A431 cells

Applicazioni

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

Risultati analitici

Negative Control
HL-60 cells

Positive Control
A-431 cells

Altre note

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable