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Key Documents

AC111S

Sigma-Aldrich

Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate

from sheep, CY5 conjugate

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

Origine biologica

sheep

Livello qualitativo

Coniugato

CY5 conjugate

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Reattività contro le specie

mouse

tecniche

immunocytochemistry: suitable

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Specificità

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

Immunogeno

Purified mouse IgG.

Applicazioni

Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate is an antibody against Sheep Mouse IgG for use in Immunocytochemistry.

Qualità

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: Performance of this Cy5-conjugated secondary antibody has been validated by indirect immunofluorescent labelling (1:250 dilution) of A431 cells following a mouse monoclonal anti-H3K27me3 primary antibody (Cat. No. 05-1951).

Descrizione del bersaglio

~150 kDa observed.

Stato fisico

Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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