ABT28

Anti-Autotaxin Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): canine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• N° accesso NCBI: NP_001035181
• N° accesso UniProt: Q13822
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ENPP2(5168)

Descrizione

Descrizione generale

Autotaxin (also known as Enpp2), an exo-enzyme that is upregulated in various cancers such as breast and lung cancer, is a secreted protein integral to the production of the lipid signaling molecule, lysophosphatidic acid (LPA). Wutotaxin is synthesized as a pre-pro-enzyme and stimulates cell proliferation, cell motility, and angiogenesis. Studies have shown increased expression of autotaxin in tumors is associated with increased tumor aggressiveness.

Specificità

This antibody recognizes Autotaxin at the substrate binding region.

Immunogeno

Epitope: Substrate binding region

KLH-conjugated linear peptide corresponding to human Autotaxin at the substrate binding region.

Applicazioni

Detect Autotaxin using this Anti-Autotaxin Antibody validated for use in WB, IH(P).

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed with Tween.

Immunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Autotaxin in human cerebral cortex and ductal carcinoma tissues.

Qualità

Evaluated by Western Blot in oligodendrocyte precursor cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Autotaxin in 10 µg of oligodendrocyte precursor cell lysate. Western Blot performed without Tween.

Descrizione del bersaglio

~100/125 kDa observed.
3 isoforms are produced by alternative splicing with molecular weights of 99, 105, and 101 kDa The calculated molecular weight of this protein is 99 kDa, but can be observed at ~125 kDa due to high glycosylation. An uncharacterized band may appear at ~35 kDa in some lysates.

Risultati analitici

Control
Oligodendrocyte precursor cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable