ABS527

Anti-Sphk2, C-term Antibody

serum, from rabbit

• Sinonimo/i: Sphingosine kinase 2, SK 2, SPK 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_001166032
• N° accesso UniProt: Q9JIA7
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Sphk2(56632)

Descrizione

Descrizione generale

Sphingosine kinase 2 (Sphk2), along with its sister enzyme Sphk1, is an important kinase needed to form the important intra and extracellular signaling lipid mediator and neuroprotective factor SPP/S1P (sphingosine 1-phosphate). Sphk2 is important in blood vessel development, brain development, as well as an anti-apoptotic factor, and it mediates many proliferation mediated responses such as those surrounding insulin and its mitogenic ability. Sphk2 is localized to the lysosome membrane primarily but can also be found in the cytoplasm. Interestingly, recent research indicates that ApoE regulates the secretion Sphingosine 1-phosphate in the brain, and SPP/S1P in turn positively regulates glutamate secretion and synaptic strength in hippocampal neurons, thus Sphk1/2 plays an important role in synaptic plasticity and neuronal activity connectivity in the brain as well.

Immunogeno

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of mouse Sphk2, C-term.

Applicazioni

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Sphk2, C-term Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of Sphk2, C-term.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Sphk2 in 10 µg of mouse lung tissue lysate.
Western Blotting Analysis: A representative lot detected Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunocytochemistry Analysis: A representative lot detected Sphk2 in Huvec cells (Venkataraman, K., et al. (2006) Biochem. J. 397:461-471).

Qualità

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Sphk2, C-term in 10 µg of RAW264.7 cell lysate.

Descrizione del bersaglio

~65 kDa observed, uncharacterized bands may be observed at higher molecular weights

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1