ABS524

Anti-Lipin-2 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Phosphatidate phosphatase LPIN2, Lipin-2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: mouse
• tecniche: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_075020
• N° accesso UniProt: Q99PI5
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Lpin2(64898)

Descrizione

Descrizione generale

Lpin2, also known as Phosphatidate phosphatase LPIN2, and encoded by the gene Lpin2, plays important roles in controlling the metabolism of fatty acids. Lpin2 acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Lpin2 also acts as a nuclear transcriptional coactivator for PGC-1α protein. Lpin2 is localized to the cytoplasm and endoplasmic reticulum membrane but is also found in the nucleus. Lpin2 is expressed in many cells and tissues including liver, lung, kidney, placenta, prostate, and intestine. Expression is also seen in RBCs and at sites of lymphopoiesis in bone and spleen. Homozygous mutations in LPIN2 result in Majeed syndrome. Majeed syndrome is an autosomal recessive, autoinflammatory disorder characterized by chronic recurrent multifocal osteomyelitis and congenital dyserythropoietic anemia.

Immunogeno

Linear peptide corresponding to mouse Lipin-2.

Applicazioni

Anti-Lipin-2 Antibody is an antibody against Lipin-2 for use in western blotting, IHC & IP.

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

Western Blotting Analysis: A representative lot detected Lipin-2 in mouse WAT cell lysate (Gropler, M. C., et al. (2008). JBC. 284(11):6763-6772).
Immunocytochemistry Analysis: A representative lot detected Lipin-2 in mouse cerebellum tissue (Mayurranjan, S., et al. (2013). PNAS. 110(2):642-647).

Qualità

Evaluated by Western Blotting in mouse hepatocytes cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lipin-2 in 10 µg of mouse hepatocytes cell lysate.

Descrizione del bersaglio

~ 110 kDa observed

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide with 50% glycerol.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2