ABS1587

Anti-Ribophorin I/RPN-I Antibody

serum, from rabbit

• Sinonimo/i: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: rat, canine, human
• Reattività contro le specie (prevista in base all’omologia): nonhuman primates (based on 100% sequence homology), porcine (based on 100% sequence homology), monkey (based on 100% sequence homology)
• tecniche: western blot: suitable
• N° accesso NCBI: NP_002941
• N° accesso UniProt: P04843
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RPN1(6184)

Descrizione

Descrizione generale

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (EC 2.4.99.18; UniProt P04843; also known as Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1) is encoded by the RPN1 gene (Gene ID 6184) in human. Ribophorin I is an ER transmembrane protein and a subunit of the mammalian Oligosaccharyltransferase (OST) complex. Ribophorin I binds newly synthesized target proteins and delivers them to the catalytic core of the OST complex. Association of ribophorin I with newly synthesized membrane proteins was observed after the exit of the nascent chain from the Sec61 translocon, and it was unaffected by the N-glycosylation status. Ribophorin I is reported to preferentially interact with misfolded over native ribonuclease A. In addition, Ribophorin I is shown to complex with another ER protein malectin and enhance malectin′s ability to prevent the secretion of the folding-defective proteins.

Immunogeno

Epitope: C-terminus.

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Ribophorin I/RPN-I.

Applicazioni

Research Category
Signaling

Research Sub Category
Organelle & Cell Markers

This Anti-Ribophorin I/RPN-I Antibody is validated for use in Western Blotting for the detection of Ribophorin I.

Western Blotting Analysis: An 1:5,000 dilution from a representative lot detected ribophorin I in in 25 µg of HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
Western Blotting Analysis: A representative lot detected ribophorin I in the alkali-extracted and Triton X-114-selected integral membrane fraction derived from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the Brij35-sensitive (BrS) and Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I associated with the rRNA-free poly(A) messenger ribonucleoproteins (mRNPs) purified by oligo(dT) from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the smooth and rough microsomes sub-fractionated from a rat liver microsome preparation (Menon, A.K., et al. (2000). Curr. Biol. 10(5):241-252).
Western Blotting Analysis: A representative lot detected ribophorin I in canine rough microsome (RM) fractions obtained by sucrose gradient centrifugation of digitonin- or DHPC-solubilized canine RM preparation with or without ribosomes stripping by EDTA treatment (Potter, M.D., and Nicchitta, C.V. (2000). J. Biol. Chem. 275(3):2037-2045).

Qualità

Evaluated by Western Blotting in canine pancreas tissue rough microsome lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Ribophorin I/RPN-I in 2.5 µL of canine pancreas tissue rough microsome lysate.

Descrizione del bersaglio

~68 kDa observed.

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable