ABS1051

Anti-Mitoferrin-1/Mfrn1 Antibody

serum, from rabbit

• Sinonimo/i: Mitoferrin-1, Mitoferrin-1/Mfrn1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: serum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse
• tecniche: western blot: suitable
• N° accesso NCBI: NP_080607
• N° accesso UniProt: Q920G8
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... Slc25A37(67712)

Descrizione

Descrizione generale

Mitoferrin-1 (UniProt Q920G8; also known as mitochondrial iron transporter 1, mitochondrial solute carrier protein, solute carrier family 25 member 37) is encoded by the Slc25a37 (also known as Mfrn, Mfrn1, Mscp) gene (Gene ID 67712) in murine species. Mitoferrin-1 and mitoferrin-2 (Slc25a28, Mfrn2) mediate iron import into mitochondria. Mitoferrin-2 is expressed ubiquitously, whereas mitoferrin-1 is specifically induced in differentiating erythroid cells. Mfrn1 knockout in zebrafish and mice disrupts heme and [Fe-S] cluster synthesis as a result of a severe reduction in mitochondrial iron in erythroid progenitors.

Specificità

This antiserum does not react with spliced isofrms 2, 3, 4, and 5.

Immunogeno

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of mouse Mitoferrin-1/Mfrn1.

Applicazioni

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Mitoferrin-1/Mfrn1 Antibody is validated for use in Western Blotting for the detection of Mitoferrin-1/Mfrn1.

Western Blotting Analysis: A 1:2,000 dilution of this antiserum detected mitoferrin-1 upregulation in mitochondria lysates from mouse erythroleukemia (MEL) cultures chemically induced to undergo erythroid maturation (Courtesy of Dr Barry H. Paw, Brigham and Women′s Hospital, Boston, MA).
Western Blotting Analysis: A representative lot detected reduced mitoferrin-1 expression due to heterozygous gene trap insertion in the Mfrn1 gene (Mfrn1+/gt) and loss of mitoferrin-1 expression due to homozygous Mfrn1 gene insertion (Mfrn1gt/gt) in mouse embryonic stem cells (mESCs) undergoing primitive erythropoiesis (Chung, J., et al.(2014). J Biol Chem. 289(11):7835-7843).
Western Blotting Analysis: A representative lot detected upregulated mitoferrin-1 expression following DMSO-induced erythroid maturation in mouse erythroleukemia (MEL) cultures (Shah, D.I., et al. (2012). Nature. 491(7425):608-612).

Qualità

Evaluated by Western Blotting in mouse embryonic stem (mES) cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected mitoferrin-1 in mouse embryonic stem (mES) cell lysates.

Descrizione del bersaglio

~34 kDa observed. Uncharacterized band(s) may appear in some lysates.

Stato fisico

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable