ABN1681

Anti-Phospho-PANX1 (Tyr198)

from rabbit

• Sinonimo/i: Pannexin-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• tecniche: immunofluorescence: suitable; western blot: suitable
• N° accesso NCBI: NP_062355
• N° accesso UniProt: Q9JIP4
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: phosphorylation (pTyr198)
• Informazioni sul gene: human ... PANX1(24145)

Descrizione

Descrizione generale

Pannexin-1 (UniProt: Q9JIP4) is encoded by the Panx1 gene (Gene ID: 55991) in murine species. Pannexin-1 is a multi-pass membrane protein of the pannexin family that serves as a high-conductance, voltage-gated channel. It is widely expressed, including in cartilage, skin, spleen, heart, and brain. In the nervous system Panx1 transcripts have been detected in the cerebellum, cortex, lens, retina, pyramidal cells, interneurons of the neocortex and hippocampus. Three pannexin family genes (PANX1-3) have been identified in murine and human genomes. Pannexins share structural and functional similarities and play important roles in several physiological processes, including ATP release, Ca2+ homeostasis, and synaptic neurotransmitters release. Pannexins can also form intermixed channels and the intermixing is more evident between Pannexin 1 and 2. The level of intermixing is governed by the degree of N-glycosylation. Post-translational N-glycosylation plays a role in cell surface targeting. Pannexin-1 can also undergo S-nitrosylation, which can inhibit channel currents and ATP release. It has been shown that Pannexin-1 channel can release ATP in response to activation by TNF-alpha that requires activation of type-1 TNF receptors and downstream signaling through Src family kinases (SFK). Tryosine 198 in the intracellular loop region of Pannexin-1 is highly conserved and is considered as a SFK phosphorylation site. Pannexin-1 has been linked to propagation of calcium waves, regulation of vascular tone, taste-bud function, and in tumor suppression in gliomas. Pannexin-1 channel opening can contribute to cell death and seizures under ischemic or epileptic conditions. (Ref.: Penuela, S et al. (2013). Biochim. Biophys. Acta 1828(11); 15-22).

Specificità

This polyclonal antibody specifically detects pannexin-1 phosphorylated on tyrosine 198 in HEK293 cells transfected with murine Pannexin 1.

Immunogeno

A linear peptide corresponding to 12 amino acids surrounding phospho-Tyr198 of murine Pannexin-1.

Epitope: cytoplasmic domain

Applicazioni

Anti-Phospho-PANX1 (Tyr198), Cat. No. ABN1681, is a highly specific rabbit polyclonal antibody that targets Pannexin 1 and has been tested in Immunofluorescence and Western blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in human coronary smooth muscle cells transfected with an epitope tagged Panx1, & recombinant human Pannexin1 and (active) human Src tyrosine kinase. (Courtesy of Isakson Lab, University of Virginia, Charlottesville, VA).

Immunofluorescence Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in isolated mesenteric venule cross-sections from mice (Lohman, A.W., et. al. (2015). Nat. Commun. 6:7965).

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in HUVECs stimulated with TNF-a (Lohman, A.W., et. al. (2015). Nat Commun. 6:7965).

Qualità

Evaluated by Western Blotting in transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-PANX1 (Tyr198) in 10 µg of lysate from HEK293 cells transfected with murine pannexin-1.

Descrizione del bersaglio

~58 kDa observed; 48.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 8.0), 0.1% BSA without azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable