ABE1851
Anti-MLL-3
from rabbit
Sinonimo/i:
HALR, Histone-lysine N-methyltransferase 2C, EC: 2.1.1.43, Lysine-N-methyltransferase 2C, Myeloid-lineage leukemia protein 3 homolog
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
rat, human, mouse
Confezionamento
antibody small pack of 25 μg
tecniche
ChIP: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... KMT2C(58508)
mouse ... Kmt2C(231051)
Descrizione generale
Histone-lysine N-methyltransferase 2D (EC 2.1.1.43; UniProt O14686; also known as MLL4, ALL1-related protein, Lysine N-methyltransferase 2D) is encoded by the KMT2D (also known as MLL4, ALR, KABUK1, MLL2) gene (Gene ID: 8085) in human. MLL4 methylates Lys-4 of histone H3 to form H3K4 mono- and di-methylation (H3K4me1/2), which represents a specific tag for transcriptional enhancers. MLL4 is also a component of the MLL3/4 protein complex that plays an important role during cell differentiation and animal development. MLL4 is localized to the nucleus and is expressed in most embryonic and adult tissues. Defects in MLL4 (often known as MLL2 and KMT2D in the literature) have been linked to Kabuki syndrome and congenital heart diseases. Inactivating mutations in MLL4/MLL2/KMT2D gene have been linked to many types of cancers, including medulloblastoma, non-Hodgkin lymphoma, diffuse large B-cell lymphoma, prostate cancer, breast cancer, hepatocellular carcinoma, ovarian cancer, lung, bladder, head & neck cancers, and pancreatic cancer. (Ref.: Lee, J.E., et al. (2013). Elife. 2:e01503; Lawrence, M.S., et al., (2014). Nature. 505(7484):495-501).
Specificità
This polyclonal antiody detects MLL-3 protein in human, mouse and rat cells. It targets and epitope within 18 amino acids from the internal region of N-terminal half.
Immunogeno
A linear peptide corresponding to 18 amino acids from the N-terminal region of human MLL-3.
Epitope: unknown
Applicazioni
Anti-MLL3, Cat. No. ABE1851, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase 2C and has been tested in Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL-3 in mouse testis, rat uterus, and mouse kidney tissues.
Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. al. (2009). Mol Cell. 36(1):75-87).
Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Chromatin Immunoprecipitation Analysis: A representative lot detected MLL-3 in MEF cells (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Western Blotting Analysis: A representative lot detected MLL-3 in Western Blotting applications (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8); Bres, V., et. al. (2009). Mol Cell. 36(1):75-87).
Immunohistochemistry Analysis: A representative lot detected MLL-3 in mouse urothelial tumors (Lee, J., et. al. (2009) Proc Natl Acad Sci USA. 106(21):8513-8).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualità
Evaluated by Western Blotting in U2OS nuclear extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected MLL-3 in 10 µg of U2OS cell nuclear extract.
Descrizione del bersaglio
~540 kDa observed; 540.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Stato fisico
Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Yingxue Wang et al.
Molecular therapy. Nucleic acids, 24, 610-621 (2021-04-27)
Papillary thyroid cancer (PTC) is the most common type of thyroid cancer, and angiogenesis plays critical roles in its recurrence and metastasis. In this study, we investigated the effects of hypoxia-induced exosomal microRNA-181 (miR-181a) from PTC on tumor growth and
Ran Chen et al.
Cell reports, 34(7), 108751-108751 (2021-02-18)
The myeloid tumor suppressor KMT2C is recurrently deleted in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), particularly therapy-related MDS/AML (t-MDS/t-AML), as part of larger chromosome 7 deletions. Here, we show that KMT2C deletions convey a selective advantage to hematopoietic
Jun-Yi Zheng et al.
Cell death & disease, 12(4), 364-364 (2021-04-08)
MLL3 is a histone H3K4 methyltransferase that is frequently mutated in cancer, but the underlying molecular mechanisms remain elusive. Here, we found that MLL3 depletion by CRISPR/sgRNA significantly enhanced cell migration, but did not elevate the proliferation rate of cancer
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