ABE1348
Anti-Histone H2A.Z Antibody (C-term)
from rabbit, purified by affinity chromatography
Sinonimo/i:
Histone H2A.Z, H2A/z
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human
tecniche
ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... H2AFZ(3015)
Descrizione generale
H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
Specificità
Recognizes histone H2A.Z
wide range expected
Immunogeno
KLH-conjugated linear peptide corresponding to region the near C-terminus of Human Histone H2A.Z.
Applicazioni
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody (1μg per reaction) was used for ChIP using chromatin from HeLa and K562 cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
ChIP-seq: A representative lot of this antibody (0.5 μg per reaction) was used to immunoprecipitate K562 chromatin for ChIP-seq analysis.
Western Blotting Analysis: A 1:2,000 dilution of a represenative lot of this antibody detected histone H2A.Z in acid extracted HeLa cell lysate.
Immunocytochemistry: A 1:500 dilution from a representative lot detected histone H2A.Z in HeLa cells.
This Anti-Histone H2A.Z Antibody (C-term) is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Immunocytochemistry and ELISA for the detection of Histone H2A.Z (C-term).
Qualità
Evaluated by Western Blotting using recombinant histones
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Western Blotting Analysis: A 1.5 µg/mL dilution of this antibody detected recombinant Histone H2A.Z, but not histones H1, H2A,H2A.X, H3, or H4.
Descrizione del bersaglio
~16 kDa observed
Stato fisico
Affinity purified rabbit polyclonal in PBS containing 0.05% azide and 0.05% ProClin 300.
Altre note
Concentration: Please refer to lot specific datasheet.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Gilda Stefanelli et al.
Cell reports, 36(7), 109551-109551 (2021-08-19)
Rapid removal of histone H2A.Z from neuronal chromatin is a key step in learning-induced gene expression and memory formation, but mechanisms underlying learning-induced H2A.Z removal are unclear. Anp32e was recently identified as an H2A.Z-specific histone chaperone that removes H2A.Z from
Guang Yang et al.
Nucleic acids research, 48(6), 3001-3013 (2020-01-23)
Nucleosomal histones are barriers to the DNA repair process particularly at DNA double-strand breaks (DSBs). However, the molecular mechanism by which these histone barriers are removed from the sites of DNA damage remains elusive. Here, we have generated a single
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