ABC457

Anti-dUTPase Antibody

from rabbit

• Sinonimo/i: Deoxyuridine 5′-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, dUTPase
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• N° accesso NCBI: NP_001020419
• N° accesso UniProt: P33316
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... DUT(1854)

Descrizione

Descrizione generale

dUTPase, also known as dUTP pyrophosphatase, and encoded by the gene DUT, is a critical enzyme involved in nucleotide metabolism for the production of dUMP, the immediate precursor of thymidine nucleotides. dUTPase normally can also function to decrease the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA and cause mutations. dUTPase is localized to the nucleus and mitochondrial locations. dUTPase is expressed in numerous tissues and has multiple isoforms. Some isoforms are constitutively expressed while others match DNA replication cycles. Because dUTPase plays a pivotal role in regulating cellular dUTP pools, this enzyme could have profound effects on the efficacy of agents that target thymidylate biosynthesis and dUTPase expression is being examined as a prognostic marker for overall survival and response to therapy in colon and other cancers where thymidylate therapy is used.

Immunogeno

Recombinant protein corresponding to human dUTPase.

Applicazioni

Anti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2 µg/mL from a representative lot detected dUTPase in 10 µg of A431, HEK293, and HeLa cell lysates and human placenta tissue lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected dUTPase in MDA-MB231, MCF-7, H184B5F5/M10 cell lysate (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

Qualità

Evaluated by Western Blotting in MDA-MB-231 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected dUTPase in 10 µg of MDA-MB-231 cell lysate.

Descrizione del bersaglio

~18 kDa observed. The expected molecular weight of this protein is 27 kDa However, this protein has been observed at 18 kDa (Prof. Z. F. Chang, National Yang-Ming University). Uncharacterized band(s) may be observed in some cell lysates.

Stato fisico

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable