AB3768
Anti-Rad54L Antibody
Chemicon®, from rabbit
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
saturated ammonium sulfate (SAS) precipitated
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, mouse
Produttore/marchio commerciale
Chemicon®
tecniche
immunocytochemistry: suitable
immunohistochemistry: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... RAD54L(8438)
Specificità
Recognizes RAD54L. The molecular weight of the protein is 84,326 Daltons.
Immunogeno
Synthetic peptide, amino acids 12-27 of RAD51L.
Applicazioni
Anti-Rad54L Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Rad54L & has been validated in ICC & IHC.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY
1. Dissect tissues and freeze on dry ice
2. Cut on a cryostat - 10um slices
3. Dry slides at room temperature for 30-90 min
4. Fix with cold Acetone/Methanol (50/50) 2 min - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin , incubation 10 minutes at 37°C, followed by 3 washes with PBS)
5. Let air dry
6. PBS 5min
7. Incubate in 50mM ammonium chloride 30 min
8. PBS 5min
9. Blocking serum 30-45min
10. Primary antibody 90min - dilution 1:100 - 1:600
11. Wash 3 times for 5 min
12. Secondary fluorescent conjug. antibody 30min
13. Wash 3 times for 5 min and mount
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.SUGGESTED PROTOCOL FOR IMMUNOHISTOCHEMISTRY
1. Dissect tissues and freeze on dry ice
2. Cut on a cryostat - 10um slices
3. Dry slides at room temperature for 30-90 min
4. Fix with cold Acetone/Methanol (50/50) 2 min - then either process further or store at -20°C . Alternative is fixation with 4% PFA and treatment with a trypsin solution. (0.05% trypsin , incubation 10 minutes at 37°C, followed by 3 washes with PBS)
5. Let air dry
6. PBS 5min
7. Incubate in 50mM ammonium chloride 30 min
8. PBS 5min
9. Blocking serum 30-45min
10. Primary antibody 90min - dilution 1:100 - 1:600
11. Wash 3 times for 5 min
12. Secondary fluorescent conjug. antibody 30min
13. Wash 3 times for 5 min and mount
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Stato fisico
Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a 1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25C° prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
Stoccaggio e stabilità
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.The rehydrated antibody solutions can be stored undiluted at 2-8C° for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8C°. For storage at -20C°, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70C° for up to 6 months. Avoid repeated freeze/thaw cycles.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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