AB1560
Anti-Opioid Receptor Antibody, δ, pain, NT
serum, Chemicon®
Sinonimo/i:
Anti-DOP, Anti-DOR, Anti-DOR1, Anti-OPRD
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
serum
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
mouse, human, rat
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Specificità
Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
Immunogeno
Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
Epitope: N-terminus
Applicazioni
Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neuroinflammation & Pain
Descrizione del bersaglio
36 and 72 kDa
Linkage
Replaces: MAB5330
Stato fisico
UnPurified whole rabbit antiSerum containing no preservatives.
Unpurified
Stoccaggio e stabilità
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Spinal cord
Spinal cord
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Comparison of immunoblotted delta opioid receptor proteins expressed in the adult rat brain and their regulation by growth hormone.
Persson, Anders I, et al.
Neuroscience Research, 52, 1-9 (2005)
Peripheral ?-opioid receptors attenuate the exercise pressor reflex.
Leal, AK; Yamauchi, K; Kim, J; Ruiz-Velasco, V; Kaufman, MP
American Journal of Physiology. Heart and Circulatory Physiology null
Glial-restricted precursors: patterns of expression of opioid receptors and relationship to human immunodeficiency virus-1 Tat and morphine susceptibility in vitro.
S K Buch, V K Khurdayan, S E Lutz, P E Knapp, N El-Hage, K F Hauser
Neuroscience null
Yuan Xu et al.
Aging, 12(24), 25035-25059 (2020-11-17)
Our previous studies have shown that the δ-opioid receptor (DOR) is an important neuroprotector via the regulation of PTEN-induced kinase 1 (PINK1), a mitochondria-related molecule, under hypoxic and MPP+ insults. Since mitochondrial dysfunctions are observed in both hypoxia and MPP+
Noufissa Kabli et al.
Pain, 127(1-2), 84-93 (2006-09-12)
The analgesic effects of local administration of opioid agonists into peripheral tissues in alleviating pain have been well documented in both clinical and preclinical studies, although few studies have examined their effects in neuropathic pain. In this study, we investigated
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.