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Sigma-Aldrich

Overnight Express™ Instant TB Medium - Novagen

Sinonimo/i:

autoinduction media 

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About This Item

Codice UNSPSC:
41106213
NACRES:
NA.54

Stato

solid

Livello qualitativo

200

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze

tecniche

microbiological culture: suitable

applicazioni

microbiology

Condizioni di spedizione

ambient

Temperatura di conservazione

10-30°C

Descrizione generale

Overnight Express™ Instant TB Medium is a complete autoinduction media in granulated terrific broth formulation. It is used in routine recombinant protein expression applications. Overnight Express Instant TB Medium is ideal for producing recombinant protein without monitoring cell growth or inducer (Isopropyl ß-D-1-thiogalactopyranoside(IPTG)) addition through lac operon-controlled Escherichia coli expression systems.

Applicazioni

Overnight Express Instant TB Medium – Novagen has been used:
  • to culture recombinant Escherichia coli colonies for protein expression.
  • as an auto-induction media to study the effect of glucose and lactose additives on recombinant ovine angiotensinogen (oANG) production
  • as a medium for hamster recombinant PrP (rPrP) protein expression

Caratteristiche e vantaggi

  • Autoinduction media: simplifies protocol by eliminating the monitoring and induction steps
  • Rich culture media: high cell densities and protein expression level
  • Granulated format: rapid and uniform dissolution in water minimize the chance of inhaling hazardous powdered ingredients
  • Singe use EasyPak packaging: Minimal sample handling significantly reduces the chance of contamination.
  • Convenient, no need to spend extra time on weighting out different ingredients

Attenzione

Toxicity: Standard Handling (A)

Nota sulla preparazione

The EasyPak aluminum foil pouch contains enough granulated medium to prepare 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and bring it to a boil in a microwave oven for 2 minutes.

Note legali

Express is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

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