71340-M

pCDFDuet-1 DNA - Novagen

• Sinonimo/i: Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• Codice UNSPSC: 41106602
• NACRES: NA.51

Proprietà

• Stato: liquid
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Promotore: Promoter name: T7
• Selezione: spectinomycin
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Applicazioni

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

Attenzione

Toxicity: Standard Handling (A)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable