71194-M
0.5 M THP Solution
Reducing agent compatible with His•Bind chromatography
Sinonimo/i:
Tris(3-hydroxypropyl)phosphine
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About This Item
Codice UNSPSC:
41106511
Prodotti consigliati
Livello qualitativo
Forma fisica
solution
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
tecniche
protein purification: suitable
applicazioni
sample preparation
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Caratteristiche e vantaggi
Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Attenzione
Toxicity: Corrosive (L)
Altre note
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Note legali
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Eye Dam. 1 - Skin Corr. 1B
Codice della classe di stoccaggio
8B - Non-combustible, corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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