70796-M

His•Tag^® Monoclonal Antibody

Novagen^®

• Sinonimo/i: Anti-His antibody
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Forma dell’anticorpo: purified antibody
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Sensitive, specific detection of His•Tag fusion proteins

The HIS TAG^® Monoclonal Antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG^® sequence encoded by many of the NOVAGEN^® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (K_d = 5 × 10^-8 to 1 × 10^-9 M) enables sensitive, specific detection of HIS TAG^® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG^® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG^® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein<TMSYMBOL></TMSYMBOL> and Trail Mix<TMSYMBOL></TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.

* Not recommended for HRP-based detection of Trail Mix Western Markers.

Attenzione

Toxicity: Standard Handling (A)

Note legali

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable