567704

Sphingolipid Ceramide N-Deacylase, Pseudomonas sp.

• Sinonimo/i: SCDase
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: bacterial (Pseudomonas spp.)
• Livello qualitativo: 100
• Forma fisica: liquid
• Attività specifica: ≥1 units/mL
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Attività estranea: α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases, none detected
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Sphingolipid ceramide N-deacylase (SCDase) is a native sphingolipid ceramide N-deacylase from Pseudomonas species. It hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in several sphingolipids and gangliosides. This enzyme catalyzes the condensation reaction between fatty acids and sphingosine bases and forms sphingolipids. It is also involved in transacylation reactions. SCDase is not recommended for hydrolysis of ceramide. It is also used to prepare lysoglycosphingolipids (lysoGSLs) effortlessly.

Applicazioni

Sphingolipid ceramide N-Deacylase, Pseudomonas sp. has been used as a PP2A enhancer to study its effects on phosphatase and tensin homolog (PTEN) in forkhead box O3 (FOXO3a) phosphorylation in primary CD4⁺ T cells.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.

Stato fisico

In 50 mM sodium acetate buffer, 0.1% LUBROL PX Detergent, pH 6.0.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52.
Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923.
Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable