344906

FURA 2/AM in Solution

• Sinonimo/i: FURA 2/AM in Solution
• Formula empirica (notazione di Hill): C₄₄H₄₇N₃O₂₄
• Numero CAS: 108964-32-5
• Peso molecolare: 1001.85
• Numero MDL: MFCD00036976
• Codice UNSPSC: 12171500
• NACRES: NA.47

Proprietà

• Saggio: ≥95% (HPLC)
• Livello qualitativo: 100
• Forma fisica: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Fluorescenza: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3; UTBYGIDPBQOHNT-UHFFFAOYSA-N

Descrizione

Descrizione generale

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

Azioni biochim/fisiol

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Irritant (B)

Stato fisico

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

Altre note

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Punto d’infiammabilità (°C): 87 °C - closed cup - (Dimethylsulfoxide)