220477-M

Chitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

• Sinonimo/i: Chitosanase, Streptomyces sp. N174, Chitosan N-acetylglucosaminohydrolase
• Numero CAS: 51570-20-8
• Classificazione EC (Enzyme Commission): 3.2.1.132 (BRENDA, IUBMB)
• Numero MDL: MFCD00211797
• Codice UNSPSC: 12352202

Proprietà

• Livello qualitativo: 100
• Forma fisica: liquid
• Attività specifica: ≥25 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 7.5 (temperature optimum 65°C for 10 minutes; recommended temperature 37°C)
• Attività estranea: chitinolytic ≤0.01%; proteases <0.25 Azocasein U/ml
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.

Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein

One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.

Stato fisico

In 50 M sodium acetate, 50% glycerol, pH 5.0.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1