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Merck
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Documenti fondamentali

219361

Sigma-Aldrich

Anti-Cathepsin D Rabbit pAb

liquid, Calbiochem®

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

saturated ammonium sulfate (SAS) precipitated

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze

Isotipo

IgG

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Anti-Cathepsin D, rabbit polyclonal, recognizes the ~50 kDa latent and the ~30 kDa active forms in breast carcinoma cell line. It is validated for ELISA, Western blotting, and immunoelectrophoresis.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.

Immunogeno

Human
purified human liver cathepsin D

Applicazioni

ELISA (≥1:1000)

Immunoelectrophoresis (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In PBS.

Ricostituzione

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Risultati analitici

Positive Control
Human liver tissue

Altre note

Monospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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M Heinrich et al.
Cell death and differentiation, 11(5), 550-563 (2004-01-24)
Acidic noncaspase proteases-like cathepsins have been introduced as novel mediators of apoptosis. A clear role for these proteases and the acidic endolysosomal compartment in apoptotic signalling is not yet defined. To understand the role and significance of noncaspases in promoting
Gonzalo A Mardones et al.
Molecular biology of the cell, 18(9), 3486-3501 (2007-06-29)
The sorting of acid hydrolase precursors at the trans-Golgi network (TGN) is mediated by binding to mannose 6-phosphate receptors (MPRs) and subsequent capture of the hydrolase-MPR complexes into clathrin-coated vesicles or transport carriers (TCs) destined for delivery to endosomes. This
Maya M Polovitskaya et al.
American journal of human genetics, 107(6), 1062-1077 (2020-11-21)
Dysfunction of the endolysosomal system is often associated with neurodegenerative disease because postmitotic neurons are particularly reliant on the elimination of intracellular aggregates. Adequate function of endosomes and lysosomes requires finely tuned luminal ion homeostasis and transmembrane ion fluxes. Endolysosomal
Rachel Allison et al.
The Journal of cell biology, 216(5), 1337-1355 (2017-04-09)
Contacts between endosomes and the endoplasmic reticulum (ER) promote endosomal tubule fission, but the mechanisms involved and consequences of tubule fission failure are incompletely understood. We found that interaction between the microtubule-severing enzyme spastin and the ESCRT protein IST1 at
Laura Pellegrini et al.
Human molecular genetics, 27(18), 3257-3271 (2018-06-20)
Mutations in leucine-rich repeat kinase 2 (LRRK2) segregate with familial Parkinson's disease (PD) and genetic variation around LRRK2 contributes to risk of sporadic disease. Although knockout (KO) of Lrrk2 or knock-in of pathogenic mutations into the mouse germline does not

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