217660

Anti-CD18 Mouse mAb (IB4)

liquid, ≥95% (SDS-PAGE), clone IB4

• Sinonimo/i: Anti-β2 Integrin
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: IB4, monoclonal
• Saggio: ≥95% (SDS-PAGE)
• Stato: liquid
• contiene: 0.5 mg/mL gentamicin sulfate as preservative
• Reattività contro le specie: human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG2a
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Anti-CD18, mouse monoclonal, clone IB4, recognizes the ~95 kDa β2 integrin subunit. It is validated for FC, IP, IHC on frozen sections, and cell inhibition assays.

Protein A purified mouse monoclonal antibody. Recognizes the ~95 kDa β2 integrin subunit (CD18).

Recognizes the ~95 kDa β2 integrin subunit. Blocks the binding of ICAM-1 and ICAM-3 to LFA-1.

Immunogeno

Human

human peripheral blood monocytes

Applicazioni

Flow Cytometry (5-10 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Cell Inhibition Assays (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Ricostituzione

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Risultati analitici

Negative Control
Nalm-6 cells

Positive Control
HPB-MLT cells

Altre note

Berman, M.E., et al. 1996. J. Immunol.156, 1515.
Kaneko, M., et al. 1995. J. Immunol. 155, 2631.
Mazzone, A., and Ricevuti, G. 1995. Haematologica80, 161.
Kaneko, M., et al. 1995. J. Immunol.155, 2631.
Landis, R.C., et al. 1994. J. Cell Biol.126, 529.
Wright, S.D., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5699.

Blocks the binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x10⁵ cultured human HPB-MLT cells using anti-CD18 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for immunoprecipitation and frozen sections. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable