217590

Anti-CD40 Mouse mAb (EA-5)

liquid, ≥95% (SDS-PAGE), clone EA-5

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: EA-5, monoclonal
• Saggio: ≥95% (SDS-PAGE)
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Anti-CD40, mouse monoclonal, clone EA-5, recognizes ~44 kDa CD40 in Raji cells. It is validated for use in Flow Cytometry, immunoprecipitation, & immunohistochemistry on frozen & paraffin sections.

Protein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.

Recognizes the ~44 kDa CD40 protein in Raji cells.

Immunogeno

transfected murine L cells expressing human CD40

Applicazioni

Flow cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

Paraffin Sections (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.

Altre note

FACS was performed on 5x10⁵ cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.

Fanslow, W.C., et al. 1992. J. Immunol.149, 655.
Law, C.L., et al. 1990. Leukemia4, 732.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable