16-199

Anti-Phosphotyrosine Antibody, recombinant clone 4G10^®, agarose conjugate

clone 4G10®, Upstate^®, from mouse

• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4G10®, monoclonal
• Reattività contro le specie: human
• Produttore/marchio commerciale: Upstate^®
• tecniche: immunoprecipitation (IP): suitable
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pTyr)
• Informazioni sul gene: human ... PID1(55022)

Descrizione

Descrizione generale

Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Specificità

Recognizes tyrosine-phosphorylated proteins from all species.

Applicazioni

Anti-Phosphotyrosine Antibody, recombinant clone 4G10, agarose conjugate detects level of Phosphotyrosine & has been published & validated for use in IP.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

Caratteristiche e vantaggi

Format: Gel Immobilized

Qualità

Routinely evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10 μL of this lot immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.

Descrizione del bersaglio

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Stato fisico

Gel Immobilized mouse monoclonal antibody covalently coupled to protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.01% Kathon, as a preservative for a total volume of 2 mL. Liquid suspension.

Protein A purified

Stoccaggio e stabilità

Stable for 6 months at 2-8ºC from date of receipt.

Risultati analitici

Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable